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KMID : 0379520070230010033
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한국독성학회지
2007 Volume.23 No. 1 p.33 ~ p.38
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Protective Effect of Carnosine Against Zn-Mediated Toxicity in Cortical Neuronal Cells
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Hue Jin-Joo
Lee Ah-Ram
Lee Yea-Eun
Cho Min-Hang
Lee Ki-Nam
Nam Sang-Yoon
Yun Young-Won
Jeong Jae-Hwang
Lee Sang-Hwa
Lee Beom-Jun
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Abstract
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Zinc is an endogenous transition metal that can be synaptically released during neuronal activity. However, zinc may contribute to the neuropathology associated with a variety of conditions. Carnosine expressed in glial cells can modulate the effects of zinc on neuronal excitability as a zinc chelator. We hypothesize that carnosine may protect against neurotoxicity of zinc in cortical neuronal cells. The cortical neuronal cells from newborn rats were prepared and exposed to zinc chloride and/or carnosine at various concentrations. Zinc at the doses of 0 to 500{mu}M decreased neuronal cell viability in a dose-dependent manner. Additionally, at the concentrations of 100 and 200{mu}M, it significantly decreased cell viability in an exposed time-dependent manner (p < 0.05). Treatment with carnosine at the concentrations of 20 and 200{mu}M significantly increased neuronal cell proliferation by approximately 14% and 20%, respectively, compared to the control (p < 0.05). At the concentrations of 100 and 200{mu}M zinc, 20{mu}M carnosine significantly increased the viability of neuronal cells by 18.3% and 12.1 %, and 200{mu}M carnosine also increased it by 33.5% and 28.6%, respectively, compared to the normal control group (p < 0.01). These results suggest that carnosine at a physiologically relevant level may protect against zinc-mediated toxicity in neuronal cells as an endogenous neuroprotective agent.
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KEYWORD
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Carnosine, Zinc, Toxicity, Neuronal cells, Cell viability
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